CEK SIMILARITY" CO-CHEMOTHERAPY TEST OF ETHYL ACETATE FRACTION OF PASAK BUMI ROOTS EXTRACT (Eurycoma longifolia Jack.) AGAINST T47D CELLS WHICH EXPOSED BY DOXORUBICIN"

Background: In an effort to cancer treatment, failure oftenoccurs primarily through chemotherapy, due to the reduce defficacy of chemotherapeutic agents because of the multi-drug resistance mechanisms, including the doxorubicin. Addition to suppress cancer cell doxorubicines also have an effect on normal cells. Additionally, doxorubicin tends to cause resistance cell against to drug. Some natural products research begin to be directed for co-chemotherapy agent with the aims for increase sensitivity, supress resistance of cancer cells and decrease of side effect posed by chemotherapy agent. Objective: The pasak bumi roots have great potential for anticancer and cochemotherapy for breast cancer. Therefore, the pasak bumi roots (Eurycoma Longifolia Jack.) can be combined with doxorubicin for drug of choice in breast cancer. Outcome: IC50 Methods: the method of this study will be performed by in vitro test. Amount of 500 g of pasak bumi root were macerated with ethanol 70 % during 24 hours and remacerated for 3 times. Fractination of extract was use ethyl acetate and aquadest. Cytotoxic test and combination test in T47D cells were used by MTT method.The concentrations of Ethyl acetate fraction used 2000 μg, 1000, 500, 250, 125, 62,5 and 31,125 μg/ml with incubation time 24 hours. And then, observed using Elisa plate reader in 595 nm. Absorbance readings were then converted into a percentage of the life of the cell to the IC50values calculated. Result: IC50 values from ethyl acetate fraction of the pasak bumi roots obtained was 340 μg/ml. The concentration of ethyl acetate fraction whichwas used for combination test were 340, 255, 170, 85 μg/ml. The combination index was 0,23682. Conclussion: This result indicate that combination of pasak bumi roots and doxorubicin have strong synegristic effect with dose selection for pasak bumi roots of 255 μg/ml and doxorubicin of 0,00193 μg/ml. Keywords : Breast Cancer T47D, Doxorubicin, Ethyl acetate fraction of pasak bumi roots, co-chemotherap